Institut Polytechnique de Paris 2 articles published in JoVE Developmental Biology Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula Arthur Boutillon1, Sophie Escot1, Nicolas B. David1 1Laboratory for Optics and Biosciences, CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris Embryonic development requires large-scale coordination of cell motion. Two-photon excitation mediated laser ablation allows the spatially controlled 3-dimensional ablation of large groups of deep cells. In addition, this technique can probe the reaction of collectively migrating cells in vivo to perturbations in their mechanical environment. Neuroscience In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes Laura Dumas*1, Solène Clavreul*2, Jason Durand1, Edwin Hernandez-Garzon3, Lamiae Abdeladim4, Raphaëlle Barry-Martinet2, Alicia Caballero-Megido1, Emmanuel Beaurepaire4, Gilles Bonvento3, Jean Livet2, Karine Loulier1 1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris Astrocytes tile the cerebral cortex uniformly, making the analysis of their complex morphology challenging at the cellular level. The protocol provided here uses multicolor labeling based on in utero electroporation to single out cortical astrocytes and analyze their volume and morphology with a user-friendly image analysis pipeline.
