INRAE, ISP, Universite de Tours 1 article published in JoVE Immunology and Infection Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies Edouard Guitton1, Arnaud Faurie1, Sebastien Lavillatte1, Thierry Chaumeil1, Pauline Gaboriaud2, Françoise Bussière2, Fabrice Laurent2, Sonia Lacroix-Lamandé2, Rodrigo Guabiraba2, Catherine Schouler2 1INRAE, PFIE, UE-1277, 2INRAE, ISP, Université de Tours Here we describe a method for the generation of germ-free chicks from eggs of a commercial broiler line, the Ross PM3. This method can be adapted for the generation of germ-free animals from other poultry species.