Inserm UMR1065 1 article published in JoVE Biology Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging Jérôme Gilleron1, Cindy Meziat1, André Sulen2, Stoyan Ivanov3, Jennifer Jager1, David Estève4, Catherine Muller4, Jean-Francois Tanti1, Mireille Cormont1 1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.
