2 articles published in JoVE
Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies Sofia Jaho1, Niels Junius1,3, Franck Borel1, Yoann Sallaz-Damaz1, Jean-Baptiste Salmon2, Monika Budayova-Spano1 1Université Grenoble Alpes, CEA, CNRS, IBS, 2CNRS, Solvay LOF, UMR 5258, Université Bordeaux, 3ELVESYS SAS This paper details the fabrication protocol of microfluidic chips developed for on-chip protein crystallization with the dialysis method and in situ X-ray diffraction experiments. The microfabrication process makes it possible to integrate a semipermeable regenerated cellulose dialysis membrane with any molecular weight cut-off, between two layers of the chip.
Optimization of Crystal Growth for Neutron Macromolecular Crystallography Elham Vahdatahar1, Niels Junius1,2, Monika Budayova - Spano1 1CEA, CNRS, IBS, Université Grenoble Alpes, 2ELVESYS SAS Structural studies of biomacromolecules by crystallography require high-quality crystals. Here we demonstrate a protocol that can be used by OptiCrys (a fully automated instrument developed in our lab) and/or microdialysis buttons for growing large high-quality crystals based on knowledge of the crystallization phase diagram.