Iconeus 1 article published in JoVE Neuroscience Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging Adrien Bertolo*1, Mohamed Nouhoum*1, Silvia Cazzanelli*1, Jeremy Ferrier*3, Jean-Charles Mariani2, Andrea Kliewer4,2, Benoit Belliard1, Bruno-Félix Osmanski3, Thomas Deffieux*1, Sophie Pezet*1, Zsolt Lenkei*2, Mickael Tanter*1 1Physics for Medicine Paris, ESPCI Paris, INSERM, CNRS, PSL Research University, 2Institute of Psychiatry and Neurosciences of Paris, INSERM U1266, Université de Paris, 3Iconeus, 4Department of Pharmacology and Toxicology, Jena University Hospital - Friedrich Schiller University Jena This protocol describes the quantification of volumetric cerebral hemodynamic variations in the mouse brain using functional ultrasound (fUS). Procedures for 3D functional activation map following sensory stimulation as well as resting-state functional connectivity are provided as illustrative examples, in anesthetized and awake mice.