Universite Paris-Saclay

4 articles published in JoVE

• Bioengineering

  
  Cell-Free Protein Synthesis from Exonuclease-Deficient Cellular Extracts Utilizing Linear DNA Templates Mahnaz Sabeti Azad*¹, Angelo Cardoso Batista*¹, Jean-Loup Faulon¹, Chase L. Beisel^2,3, Jerome Bonnet⁴, Manish Kushwaha^{1 1}INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, ²Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), ³Medical Faculty, University of Würzburg, ⁴Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier Presented here is a protocol for the preparation and buffer calibration of cell extracts from exonuclease V knockout strains of Escherichia coli BL21 Rosetta2 (ΔrecBCD and ΔrecB). This is a fast, easy, and direct approach for expression in cell-free protein synthesis systems using linear DNA templates.

• Engineering

  
  Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress Kevin Baudry^1,2,3, Christine Paysant-Le Roux^1,2, Stefano Colella⁴, Benoît Castandet^1,2, Marie-Laure Martin^{1,2,5 1}Université Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2), Orsay, France, ²Université de Paris, CNRS, INRAE, Institute of Plant Sciences Paris Saclay (IPS2), Orsay, France, ³Université Paris-Saclay, INRAE, CNRS, AgroParisTech, GQE - Le Moulon, Gif-sur-Yvette, France, ⁴LSTM, Univ Montpellier, INRAE, IRD, CIRAD, Institut Agro, Montpellier, France, ⁵Universitté Paris-Saclay, AgroParisTech, INRAE, UMR MIA-Paris, Paris, France DiCoExpress is a script-based tool implemented in R to perform an RNA-Seq analysis from quality control to co-expression. DiCoExpress handles complete and unbalanced design up to 2 biological factors. This video tutorial guides the user through the different features of DiCoExpress.

• Neuroscience

  
  Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy Martina Marinello*^1,2, Jérémie Cosette*¹, Caroline Bogni^1,2, Jérôme Denard^1,2, Daniel Stockholm*^1,3, Ana Buj-Bello*^{1,2 1}Genethon, 91000, Evry, France, ²Université Paris-Saclay, Univ Evry, Inserm, Genethon, Integrare research unit UMR_S951, 91000, Evry, France, ³Ecole Pratique des Hautes Etudes, PSL Research University, 75014, Paris, France This protocol describes a method for morphometric analysis of neuromuscular junctions by combined confocal and STED microscopy that is used to quantify pathological changes in mouse models of SMA and ColQ-related CMS.

• Biology

  
  Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe Aline Stedman^1,3, Antonin Levy^1,4, Philippe J. Sansonetti^1,2,5, Giulia Nigro^{1,6 1}Molecular Microbial Pathogenesis Unit, Institut Pasteur, ²Chaire de Microbiologie et Maladies Infectieuses, Collège de France, ³Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, Sorbonne Université, CNRS UMR7622, INSERM U1156, ⁴Molecular Radiotherapy, INSERM U1030, Gustave Roussy, Université Paris-Saclay, ⁵The Center for Microbes, Development and Health, Institut Pasteur Shanghai and Chinese Academy of Sciences, ⁶Microenvironment and Immunity Unit, Institut Pasteur The present protocol describes a method to detect reactive oxygen species (ROS) in the intestinal murine organoids using qualitative imaging and quantitative cytometry assays. This work can be potentially extended to other fluorescent probes to test the effect of selected compounds on ROS.