Universite de Bourgogne Franche-Comte

1 article published in JoVE

• Biology

  
  Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets Geetika Raizada¹, Balasubramaniam Namasivayam², Sameh Obeid³, Benjamin Brunel¹, Wilfrid Boireau¹, Eric Lesniewska⁴, Celine Elie-Caille^{1 1}Université de Franche-Comté, CNRS UMR-6174, FEMTO-ST Institute, ²Lille Neuroscience & Cognition research centre, ³Institut Galien Paris-Saclay, CNRS UMR-8612 , Université Paris-Saclay, ⁴Interdisciplinary Lab Carnot Bourgogne LICB, CNRS UMR-6303, Université de Bourgogne Franche-Comté This paper proposes a new generation of multiparametric analytical platforms with increased throughput for the characterization of extracellular vesicle subsets. The method is based on a combination of multiplexed biosensing methods with metrological and morphomechanical analyses by atomic force microscopy, coupled with Raman spectroscopy, to qualify vesicular targets trapped on a microarray biochip.