3 articles published in JoVE
Sit-to-stand-and-walk from 120% Knee Height: A Novel Approach to Assess Dynamic Postural Control Independent of Lead-limb Gareth D. Jones1,2, Darren C. James3, Michael Thacker1,2, David A. Green1 1 Here, we present a novel protocol to measure positional stability at key events during the sit-to-stand-to-walk using the center-of-pressure to the whole-body-center-of-mass distance. This was derived from the force platform and three-dimensional motion-capture technology. The paradigm is reliable and can be utilized for the assessment of neurologically compromised individuals.
Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants Joo Wook Ahn1, Michael Coldwell2, Susan Bint2, Caroline Mackie Ogilvie1 1Cytogenetics Department, Guy's & St Thomas' NHS Foundation Trust, 2Cytogenetics Department, Viapath Analytics Array CGH for the detection of genomic copy number variants has replaced G-banded karyotype analysis. This paper describes the technology and its application in a diagnostic service laboratory.
FISH for Pre-implantation Genetic Diagnosis Paul N. Scriven1, Toby L. Kirby1, Caroline Mackie Ogilvie1 1Department of Cytogenetics, GSTS-Pathology, Guy’s & St Thomas’ NHS Foundation Trust, Guy’s & St Thomas’ Centre for Preimplantation Genetic Diagnosis This article describes the selection of suitable probes for single-cell FISH, spreading techniques for blastomere nuclei, and in situ hybridization and signal scoring, applied to pre-implantation genetic diagnosis (PGD) in a clinical setting.