3 articles published in JoVE
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies Tim M. Curtis*1, Declan McLaughlin2, Michael O'Hare1, Joanna Kur1, Peter Barabas1, Gordon Revolta1, C. Norman Scholfield3, J. Graham McGeown4, Mary K. McGahon*2 1 This manuscript describes a straightforward protocol for the isolation of arterioles from the rat retina that can be used in electrophysiological, calcium imaging and pressure myography studies.
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions Myriam Jaraíz-Rodríguez1, Ana González-Sánchez1,2, Laura García-Vicente1, Jose M. Medina1, Arantxa Tabernero1 1Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, 2 This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.
Preparation of Highly Coupled Rat Heart Mitochondria Irina Gostimskaya1, Alexander Galkin2 1Faculty of Life Sciences, University of Manchester, 2School of Biological Sciences, Queen's University Belfast We describe а protocol for isolation of pure, highly coupled rat heart mitochondria for functional or structural studies of cellular bioenergetics, biophysical measurements, proteomics or mitochondrial DNA and lipids analysis.