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3 articles published in JoVE
Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq George E. Gentsch1, Ilya Patrushev1, James C. Smith1 1Division of Systems Biology, MRC National Institute for Medical Research The question of how chromatin regulators and chromatin states affect the genome in vivo is key to our understanding of how early cell fate decisions are made in the developing embryo. ChIP-Seq—the most popular approach to investigate chromatin features at a global level—is outlined here for Xenopus embryos.
Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation Emilie Pacary1, Matilda A. Haas1, Hendrik Wildner1, Roberta Azzarelli1, Donald M. Bell2, Djoher Nora Abrous3, François Guillemot1 1Division of Molecular Neurobiology, MRC National Institute for Medical Research, 2Confocal and Image Analysis Laboratory, National Institute for Medical Research, 3Physiopathologie de la plasticité neuronale, Neurocentre Magendie, Université de Bordeaux This article describes in detail a protocol to electroporate in utero the cerebral cortex and the hippocampus at E14.5 in mice. We also show that this is a valuable method to study dendrites and spines in these two cerebral regions.