University of Warwick View Institution's Website 10 articles published in JoVE Engineering Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis Ateeq ur Rehman1, Rahman S. Zabibah2, Samira Kharratian3, Adil Mustafa4,5 1Department of Biomedical Engineering, Foundation University Islamabad, 2College of Medical Technology, The Islamic University Najaf, 3Faculty of Mechanical Engineering, University of Tabriz, 4Department of Engineering Mathematics, University of Bristol, 5School of Life Sciences, University of Warwick Breast cancer cells exhibit different dielectric properties compared to non-tumor breast epithelial cells. It has been hypothesized that, based on this difference in dielectric properties, the two populations can be separated for immunotherapy purposes. To support this, we model a microfluidic device to sort MCF-7 and MCF-10A cells. Biology Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers Darius Vasco Köster1, Abrar Bhat2, Sankarshan Talluri2, Satyajit Mayor2 1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, 2National Centre for Biological Sciences, Tata Institute of Fundamental Research This protocol describes the formation of supported lipid bilayers and the addition of cytoskeletal filaments and motor proteins to study the dynamics of reconstituted, membrane-tethered cytoskeletal networks using fluorescence microscopy. Immunology and Infection Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung Niamh E. Harrington*1, Esther Sweeney*1, Ilyas Alav1,2, Freya Allen1, John Moat1,3, Freya Harrison1 1School of Life Science, University of Warwick, 2Institute of Microbiology and Infection, University of Birmingham, 3Warwick Antimicrobial Screening Facility, School of Life Science, University of Warwick This workflow can be used to perform antibiotic susceptibility testing using an established ex vivo model of bacterial biofilm in the lungs of individuals with cystic fibrosis. Use of this model could enhance the clinical validity of MBEC (minimal biofilm eradication concentration) assays. Bioengineering Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement Jun Liu1, John Wilson2, Claire Davis1, Anthony Peyton2 1Advanced Steel Research Centre, Warwick Manufacturing Group, University of Warwick, 2School of Electrical and Electronic Engineering, University of Manchester This paper elaborates the sample and sensor preparation procedures and the protocols for using the test rig particularly for dynamic domain imaging with in situ BH measurements in order to achieve optimal domain pattern quality and accurate BH measurements. Engineering Inkjet-printed Polyvinyl Alcohol Multilayers Iulia Salaoru1,2, Zuoxin Zhou2, Peter Morris3, Gregory J. Gibbons2 1Emerging Technologies Research Centre (EMTERC), De Montfort University, 2WMG, University of Warwick, 3PVOH Polymer Ltd. An inkjet printer was used to manufacture polyvinyl alcohol multilayers. Polyvinyl alcohol water-based ink was formulated, and the main physical properties were investigated. Engineering Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells Ruth Cherrington1, Benjamin Michael Wood1, Iulia Salaoru1, Vannessa Goodship1 1Warwick Manufacturing Group, University of Warwick This paper investigates the suitability of inkjet printing for the manufacturing of dye-sensitized solar cells. A binder-free TiO2 nanoparticle ink was formulated and printed onto a FTO glass substrate. The printed layer was fabricated into a cell with an active area of 0.25 cm2 and an efficiency of 3.5%. Engineering Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems David C. Smith1, Joseph H. Spencer1, Jeremy Sloan2, Liam P. McDonnell1, Harrison Trewhitt2, Reza J. Kashtiban2, Eric Faulques3 1School of Physics and Astronomy, University of Southampton, 2Department of Physics, University of Warwick, 3Institut des Matériaux Jean Rouxel, University of Nantes CNRS The paper describes a method for producing extreme nanowires by melt infiltration into carbon nanotubes and how 1D systems may be characterized and investigated using Resonance Raman Spectroscopy to determine vibrational and optical excitation energies. Chemistry Assessment of Boron Doped Diamond Electrode Quality and Application to In Situ Modification of Local pH by Water Electrolysis Tania L. Read1, Julie V. Macpherson1 1Warwick Electrochemistry and Interfaces Group, Department of Chemistry, University of Warwick A protocol is described for the characterization of the key electrochemical parameters of a boron doped diamond (BDD) electrode and subsequent application for in situ pH generation experiments. Biology Identification of Post-translational Modifications of Plant Protein Complexes Sophie J. M. Piquerez1, Alexi L. Balmuth2, Jan Sklenář2, Alexandra M.E. Jones1,2, John P. Rathjen3, Vardis Ntoukakis1 1School of Life Sciences, University of Warwick, 2The Sainsbury Laboratory, Norwich Research Park, 3Research School of Biology, The Australian National University We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners. Biology Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting Jesper T. Grønlund1, Alison Eyres1, Sanjeev Kumar1, Vicky Buchanan-Wollaston1,2, Miriam L. Gifford1,2 1School of Life Sciences, University of Warwick, 2Warwick Systems Biology, University of Warwick A method for producing Arabidopsis leaf protoplasts that are compatible with fluorescence activated cell sorting (FACS), allowing for studies of specific cell populations. This method is compatible with any Arabidopsis line that expresses GFP in a subset of cells.