3 articles published in JoVE
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092 Simone Culurgioni1,2, Minzhe Tang1,2, David R. Hall1, Martin A. Walsh1,2 1Diamond Light Source, Harwell Science & Innovation Campus, 2Research Complex at Harwell, Harwell Science & Innovation Campus A streamlined protocol for performing an extensive biochemical and structural characterization of a carbohydrate substrate binding protein from Streptococcus pneumoniae is presented.
Volume Segmentation and Analysis of Biological Materials Using SuRVoS (Super-region Volume Segmentation) Workbench Michele C. Darrow1, Imanol Luengo1,2, Mark Basham1, Matthew C. Spink1, Sarah Irvine1, Andrew P. French2, Alun W. Ashton1, Elizabeth M.H. Duke1 1Science Division, Harwell Science and Innovation Campus, Diamond Light Source, 2School of Computer Science, University of Nottingham Segmentation of three-dimensional data from many imaging techniques is a major bottleneck in analysis of complex biological systems. Here, we describe the use of SuRVoS Workbench to semi-automatically segment volumetric data at various length-scales using example datasets from cryo-electron tomography, cryo soft X-ray tomography, and phase contrast X-ray tomography techniques.
Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures Sergey V. Borisenko1, Volodymyr B. Zabolotnyy1, Alexander A. Kordyuk1,2, Danil V. Evtushinsky1, Timur K. Kim1,3, Emanuela Carleschi4, Bryan P. Doyle4, Rosalba Fittipaldi5, Mario Cuoco5, Antonio Vecchione5, Helmut Berger6 1Institute for Solid State Research, IFW-Dresden, 2Institute of Metal Physics of National Academy of Sciences of Ukraine, 3Diamond Light Source LTD, 4Department of Physics, University of Johannesburg, 5CNR-SPIN, and Dipartimento di Fisica "E. R. Caianiello", Università di Salerno, 6Institute of Physics of Complex Matter, École Polytechnique Fédérale de Lausanne The overall goal of this method is to determine the low-energy electronic structure of solids at ultra-low temperatures using Angle-Resolved Photoemission Spectroscopy with synchrotron radiation.