2 articles published in JoVE
Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems Jean-Philippe Mocho1, Darren J. Martin1, Mollie E. Millington1, Yolanda Saavedra Torres1 1Biological Research Facility, The Francis Crick Institute This protocol describes the use of sump swabs and sludge analysis of zebrafish systems, which leads to increased detection compared to the sole use of sentinels to detect pathogens such as Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa. A system to monitor P. tomentosa eggs in quarantine is also proposed.
Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging Diana Passaro*1, Ander Abarrategi*1, Katie Foster1, Linda Ariza-McNaughton1, Dominique Bonnet1 1Haematopoietic Stem Cell Laboratory, The Francis Crick Institute A method to create and live-image different humanized bone-marrow niches in mice is presented. Based on the supportive niche created by human mesenchymal cells, the addition of human endothelial cells induces the formation of human vessels, while the addition of rhBMP-2 induces the formation of human-mouse chimeric mature bone tissue.