Research Complex at Harwell

2 articles published in JoVE

• Biochemistry

  
  Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092 Simone Culurgioni^1,2, Minzhe Tang^1,2, David R. Hall¹, Martin A. Walsh^{1,2 1}Diamond Light Source, Harwell Science & Innovation Campus, ²Research Complex at Harwell, Harwell Science & Innovation Campus A streamlined protocol for performing an extensive biochemical and structural characterization of a carbohydrate substrate binding protein from Streptococcus pneumoniae is presented.

• Biology

  
  Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli Louise E. Bird¹, Heather Rada¹, Anil Verma¹, Raphael Gasper^2,3, James Birch⁴, Matthew Jennions⁴, Jan Lӧwe³, Isabel Moraes⁴, Raymond J. Owens^{1 1}Oxford Protein Production Facility, Research Complex at Harwell, ²Protein Crystallography Group, Ruhr University, ³MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, ⁴Membrane Protein Laboratory, Diamond Light Source A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.