3 articles published in JoVE
Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology Fabrice Gorrec1, Jan Löwe1 1Laboratory of Molecular Biology, Medical Research Council Automated systems and protocols for the routine preparation of a large number of screens and nanoliter crystallization droplets for vapor diffusion experiments are described and discussed.
Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR) Priya Crosby1, Nathaniel P. Hoyle1, John S. O'Neill1 1MRC Laboratory of Molecular Biology Genetically encoded luciferase is a popular non-invasive reporter of gene expression. Use of an automated longitudinal luciferase imaging gas- and temperature-optimized recorder (ALLIGATOR) enables longitudinal recording from bioluminescent cells under a wide range of conditions. Here we show how ALLIGATOR may be used in the context of circadian rhythms research.
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli Louise E. Bird1, Heather Rada1, Anil Verma1, Raphael Gasper2,3, James Birch4, Matthew Jennions4, Jan Lӧwe3, Isabel Moraes4, Raymond J. Owens1 1Oxford Protein Production Facility, Research Complex at Harwell, 2Protein Crystallography Group, Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory, Diamond Light Source A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.