National Physical Laboratory 4 articles published in JoVE Medicine A Stable Phantom Material for Optical and Acoustic Imaging Lina Hacker1,2, Aoife M. Ivory3, James Joseph4,5, Janek Gröhl1,2, Bajram Zeqiri3, Srinath Rajagopal3, Sarah E. Bohndiek1,2 1Department of Physics, University of Cambridge, 2Cancer Research UK Cambridge Institute, University of Cambridge, 3Ultrasound and Underwater Acoustics Group, Department of Medical, Marine and Nuclear Physics, National Physical Laboratory, 4School of Science and Engineering, University of Dundee, 5Centre for Medical Engineering and Technology, University of Dundee This protocol describes the fabrication of a stable, biologically relevant phantom material for optical and acoustic biomedical imaging applications, featuring independently tunable acoustic and optical properties. Biochemistry High-Throughput Protein Crystallization via Microdialysis Tristan O. C. Kwan1, Amy E. Danson1, Pascelle Draper2, Paul Reardon2, Isabel Moraes1 1National Physical Laboratory, 2SWISSCI LTD The presented protocol describes a straightforward approach for screening protein crystallization conditions and crystal growth using a 96-well high-throughput dialysis plate. The use of dialyzer tubes for the large-scale growth of microcrystals is also demonstrated for serial crystallography and MicroED applications. Environment Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization Inder Kaur1, Laura-Jayne Ellis1, Isabella Romer1, Ratna Tantra2, Marie Carriere3,4, Soline Allard5, Martine Mayne-L'Hermite5, Caterina Minelli6, Wolfgang Unger7, Annegret Potthoff8, Steffi Rades7, Eugenia Valsami-Jones1 1School of Geography, Earth and Environmental Sciences, University of Birmingham, 2Analytical Science, National Physical Laboratory, 3INAC-LCIB, Université Grenoble Alpes, 4CEA, INAC-SyMMES, 5NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay, 6Chemical, Medical and Environmental Science, National Physical Laboratory, 7BAM Division 6.1 'Surface Analysis and Interfacial Chemistry', BAM Federal Institute for Materials Research and Testing, 8Fraunhofer Institute for Ceramic Technologies and Systems Here, we present a step-wise protocol for the dispersion of nanomaterials in aqueous media with real-time characterization to identify the optimal sonication conditions, intensity, and duration for improved stability and uniformity of nanoparticle dispersions without impacting the sample integrity. Biology Test Samples for Optimizing STORM Super-Resolution Microscopy Daniel J. Metcalf1, Rebecca Edwards1, Neelam Kumarswami1, Alex E. Knight1 1Analytical Science Division, National Physical Laboratory We describe the preparation of three test samples and how they can be used to optimize and assess the performance of STORM microscopes. Using these examples we show how to acquire raw data and then process it to acquire super-resolution images in cells of approximately 30-50 nm resolution.
