2 articles published in JoVE
Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies I-Ning Lee*1,2, Joseph Hosford*1, Shuai Wang3, John A. Hunt4, Judith M. Curran2, William P. Heath3, Lu Shin Wong1 1Manchester Institute of Biotechnology & School of Chemistry, University of Manchester, 2School of Engineering, University of Liverpool, 3School of Electrical and Electronic Engineering, University of Manchester, 4School of Science and Technology, Nottingham Trent University Here we present a protocol for wide-area scanning probe nanolithography enabled by the iterative alignment of probe arrays, as well as the utilization of lithographic patterns for cell-surface interaction studies.
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids Kelly Yarnell1, Rebecca S. Purcell2, Susan L. Walker2 1School of Animal Rural and Environmental Science, Nottingham Trent University, 2Chester Zoo, Upton-by-Chester Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. The method offers a non-invasive option to assess long term patterns in both domestic and free ranging horses. This protocol describes the enzyme linked immunoassay involved and the associated biochemical validation.