3 articles published in JoVE
Controllable Nucleation of Cavitation from Plasmonic Gold Nanoparticles for Enhancing High Intensity Focused Ultrasound Applications James R. McLaughlan1,2 1School of Electronic and Electrical Engineering, University of Leeds, 2Leeds Institute of Cancer and Pathology, University of Leeds This protocol demonstrates the controllable nucleation of cavitation in gel phantoms, through simultaneous exposure to both near-infrared pulsed laser light and high intensity focused ultrasound (HIFU). The cavitation activity can then be used for enhancing imaging and/or therapeutic uses of HIFU.
Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems Charlotte A. Scarff1, Martin J. G. Fuller2, Rebecca F. Thompson2, Matthew G. Iadaza1 1Astbury Centre for Structural Molecular Biology, University of Leeds, 2Astbury Biostructure Laboratory, University of Leeds Negative stain EM is a powerful technique for visualizing macromolecular structure, but different staining techniques can produce varying results in a sample dependent manner. Here several negative staining approaches are described in detail to provide an initial workflow for tackling the visualization of challenging systems.
Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes Yogita Patil-Sen1, Amin Sadeghpour2, Michael Rappolt2, Chandrashekhar V. Kulkarni1 1Centre for Materials Science, School of Physical Sciences and Computing, University of Central Lancashire, 2School of Food Science & Nutrition, University of Leeds We report on a smart application of carbon nanotubes for kinetic stabilization of lipid particles that contain self-assembled nanostructures in their cores. The preparation of lipid particles requires rather low concentrations of carbon nanotubes permitting their use in biomedical applications such as drug delivery.