3 articles published in JoVE
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions Stefan Schoenfelder*1, Biola-Maria Javierre*1,2, Mayra Furlan-Magaril1,3, Steven W. Wingett1,4, Peter Fraser1,5 1Nuclear Dynamics Programme, The Babraham Institute, Babraham Research Campus, 2IJC Building, Campus ICO-Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, 3Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 4Bioinformatics Group, The Babraham Institute, Babraham Research Campus, 5Department of Biological Science, Florida State University DNA regulatory elements, such as enhancers, control gene expression by physically contacting target gene promoters, often through long-range chromosomal interactions spanning large genomic distances. Promoter Capture Hi-C (PCHi-C) identifies significant interactions between promoters and distal regions, enabling the assignment of potential regulatory sequences to their target genes.
Robust 3D DNA FISH Using Directly Labeled Probes Daniel J. Bolland1, Michelle R. King2, Wolf Reik2,3, Anne E. Corcoran1, Christel Krueger2 1Nuclear Dynamics Programme, The Babraham Institute, 2Epigenetics Programme, The Babraham Institute, 3Centre for Trophoblast Research, University of Cambridge We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond Eleftherios Karanasios1, Eloise Stapleton2, Simon A. Walker1, Maria Manifava1, Nicholas T. Ktistakis1 1Signalling Programme, The Babraham Institute, 2MRC Group, Cardiff School of Biosciences, Cardiff University Time-lapse microscopy of fluorescently labeled autophagy markers allows monitoring of the dynamic autophagy response with high temporal resolution. Using specific autophagy and organelle markers in a combination of 3 different colors, we can follow the contribution of a protein to autophagosome formation in a robust spatial and temporal context.