Institute of Cancer Research 4 articles published in JoVE Genetics A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes Christoph N. Schlaffner1,2,3, Georg J. Pirklbauer2, Andreas Bender3, Judith A.J. Steen1, Jyoti S. Choudhary2,4 1 Here we present the proteogenomic tool PoGo and protocols for fast, quantitative, post-translational modification and variant enabled mapping of peptides identified through mass spectrometry onto reference genomes. This tool is of use to integrate and visualize proteogenomic and personal proteomic studies interfacing with orthogonal genomics data. Cancer Research Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures Eamonn Morrison1,2, Patty Wai1,2, Andri Leonidou1,2, Philip Bland1,2, Saira Khalique1,2, Gillian Farnie3, Frances Daley1, Barrie Peck1,2, Rachael Natrajan1,2 1The Breast Cancer Now Toby Robins Research Centre, Division of Breast Cancer, The Institute of Cancer Research, 2Division of Molecular Pathology, The Institute of Cancer Research, 3Institute of Cancer Sciences, University of Manchester Identifying novel drug targets that transition from pre-clinical testing to human trials is a scientific priority. To that end, here we describe a functional genomics approach for examining the impact of gene depletion on cancer cell line spheroids, which more appropriately model human cancers in vivo. Medicine Three-Dimensional (3D) Tumor Spheroid Invasion Assay Maria Vinci1,2, Carol Box2, Suzanne A. Eccles2 1Division of Molecular Pathology, The Institute of Cancer Research, 2Division of Cancer Therapeutics, The Institute of Cancer Research Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers. Medicine Voluntary Breath-hold Technique for Reducing Heart Dose in Left Breast Radiotherapy Frederick R. Bartlett1, Ruth M. Colgan1, Ellen M. Donovan1, Karen Carr1, Steven Landeg1, Nicola Clements1, Helen A. McNair1, Imogen Locke1, Philip M. Evans2, Joanne S. Haviland3, John R. Yarnold4, Anna M. Kirby1 1Department of Radiotherapy, Royal Marsden NHS Foundation Trust, 2Centre for Vision, Speech and Signal Processing, Faculty of Engineering and Physical Sciences, University of Surrey, 3Clinical Trials and Statistics Unit (ICR-CTSU), Institute of Cancer Research, Sutton, UK, 4Division of Radiotherapy and Imaging, Institute of Cancer Research, Sutton, UK The current priority in breast cancer radiotherapy is to reduce cardiac doses without compromising target tissue coverage. The voluntary breath-hold technique described here is a simple, inexpensive solution to this problem and capable of being instituted widely without the need for specialized equipment.
