3 articles published in JoVE
An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay Lien Anh Ha Do1,2, Reuben Tse1, Jordan Nathanielsz1, Jeremy Anderson1, Darren Suryawijaya Ong1, Keith Chappell3, Kim Mulholland1,2,4, Paul V. Licciardi1,2 1 This study describes a high throughput, imaging-based micro-neutralization assay to determine the titer of neutralizing antibodies specific for respiratory syncytial virus (RSV). This assay format has been tested on different sample types.
Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis Hollie Burrell-Saward1, Theresa H. Ward1 1Department of Infection and Immunity, London School of Hygiene & Tropical Diseases This manuscript describes the use of a bioluminescent strain of African trypanosomes to enable the tracking of late stage infection and demonstrates how in vivo live imaging can be used to visualize infections within the central nervous system in real-time.
Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model Neveda Naz1, Dominic C. Mills1, Brendan W. Wren1, Nick Dorrell1 1Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine Performing cell culture assays for investigating bacterial adhesion and invasion under aerobic conditions is usually unrepresentative of the in vivo environment. A Vertical Diffusion Chamber model allows study of interactions of the human pathogen Campylobacter jejuni with intestinal epithelial cells under more in vivo-like conditions, resulting in enhanced bacterial invasion.