3 articles published in JoVE
Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function Yogesh Singh1,2, Oliver A. Garden3, Florian Lang2, Bradley S. Cobb1 1Department of Comparative Biomedical Sciences, The Royal Veterinary College, 2Institute of Physiology I, Cardiology & Vascular Medicine, Eberhard Karls University of Tuebingen, 3Department of Clinical Science and Services, The Royal Veterinary College Many experimental systems have been utilized to understand the mechanisms regulating T cell development and function in an immune response. Here a genetic approach using retroviral transduction is described, which is economic, time efficient, and most importantly, highly informative in identifying regulatory pathways.
In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy Jayesh Dudhia1, Patricia Becerra2, Miguel A. Valdés2, Francisco Neves2, Neil G. Hartman3, Roger K.W. Smith1 1Department of Clinical Science and Services, The Royal Veterinary College, 2Hospital de Referencia La Equina, Manilva, 3Department of Nuclear Medicine, Barts & The London NHS Trust This protocol describes the radiolabeling of equine mesenchymal stem cells and their implantation into tendon injuries in the horse in order to determine cell survival and tissue distribution using gamma scintigraphy.
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens Christopher P. Barkway1, Rebecca L. Pocock1, Vladimir Vrba2, Damer P. Blake1 1Department of Pathology and Pathogen Biology, Royal Veterinary College, London, 2BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.