Chester Zoo 1 article published in JoVE Biology Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids Kelly Yarnell1, Rebecca S. Purcell2, Susan L. Walker2 1School of Animal Rural and Environmental Science, Nottingham Trent University, 2Chester Zoo, Upton-by-Chester Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. The method offers a non-invasive option to assess long term patterns in both domestic and free ranging horses. This protocol describes the enzyme linked immunoassay involved and the associated biochemical validation.