Liverpool John Moores University 1 article published in JoVE Bioengineering Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture Michael P. Storm1, Ian Sorrell2, Rebecca Shipley3, Sophie Regan2, Kim A. Luetchford1, Jean Sathish2, Steven Webb4, Marianne J. Ellis1 1Department of Chemical Engineering and Centre for Regenerative Medicine, University of Bath, 2MRC Centre for Drug Safety Science and Institute of Translational Medicine, University of Liverpool, 3Mechanical Engineering, University College London, 4Department of Applied Mathematics, Liverpool John Moores University The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments16-21. This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.
