University of Glasgow

11 articles published in JoVE

• Medicine

  
  Destabilization of the Medial Meniscus and Cartilage Scratch Murine Model of Accelerated Osteoarthritis Lynette Dunning¹, Kendal McCulloch¹, John C. Lockhart¹, Carl S. Goodyear², Carmen Huesa^{2 1}Institute of Biomedical and Environmental Health Research, University of the West of Scotland, ²Institute of Infection, Immunity and Inflammation, University of Glasgow The present protocol describes the controlled microblade scratches on the surface of the articular cartilage after destabilizing the mouse knee by cutting the medial miniscotibial ligament. This animal model presents an accelerated form of osteoarthritis (OA) suitable for studying osteophyte formation, osteosclerosis, and early-stage pain.

• Medicine

  
  Interventional Diagnostic Procedure: A Practical Guide for the Assessment of Coronary Vascular Function Daniel T. Y. Ang^1,2, Novalia P. Sidik^1,2, Andrew J. Morrow^1,2, Robert Sykes^1,2, Margaret B. McEntegart¹, Colin Berry^{1,2 1}Department of Cardiology, Golden Jubilee National Hospital, ²British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow The purpose of this practical guide is to provide information on the preparation and administration of an interventional diagnostic procedure in clinical practice. It discusses some key preparation and safety considerations, as well as tips for procedural success.

• Bioengineering

  
  Experimental and Data Analysis Workflow for Soft Matter Nanoindentation Giuseppe Ciccone¹, Mariana Azevedo Gonzalez Oliva¹, Nelda Antonovaite², Ines Lüchtefeld³, Manuel Salmeron-Sanchez¹, Massimo Vassalli^{1 1}Centre for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, ²Optics 11 life, ³Laboratory of Biosensors and Bioelectronics, ETH Zürich The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. First, the experimental steps to acquire force spectroscopy data are detailed; then, the analysis of such data is detailed through a newly developed open-source Python software, which is free to download from GitHub.

• Immunology and Infection

  
  A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) Wilber Sabiiti¹, Bariki Mtafya^1,2, Daniela Alferes De Lima¹, Evelin Dombay¹, Vincent O. Baron¹, Khalide Azam³, Katarina Oravcova⁴, Derek J. Sloan¹, Stephen H. Gillespie^{1 1}Division of Infection and Global Health, School of Medicine, University of St Andrews, ²National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, ³Instituto Nacional de Saúde (INS), Ministério da Saúde, ⁴Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests.

• Cancer Research

  
  Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking Jodie Hay*¹, Michael W. Moles*¹, Jennifer Cassels¹, Alison M. Michie^{1 1}Paul O'Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow This protocol enables the optimization and subsequent efficient generation of nuclear and cytoplasmic fractions from primary chronic lymphocytic leukemia cells. These samples are used to determine protein localization as well as changes in protein trafficking that take place between the nuclear and cytoplasmic compartments upon cell stimulation and drug treatment.

• Engineering

  
  Generation and Coherent Control of Pulsed Quantum Frequency Combs Benjamin MacLellan*¹, Piotr Roztocki*¹, Michael Kues^1,2, Christian Reimer¹, Luis Romero Cortés¹, Yanbing Zhang¹, Stefania Sciara^1,3, Benjamin Wetzel^1,4, Alfonso Cino³, Sai T. Chu⁵, Brent E. Little⁶, David J. Moss⁷, Lucia Caspani⁸, José Azaña¹, Roberto Morandotti^{1,9,10 1}Institut National de la Recherche Scientifique - Centre Énergie, Matériaux et Télécommunications (INRS-EMT), ²School of Engineering, University of Glasgow, ³Department of Energy, Information Engineering and Mathematical Models, University of Palermo, ⁴School of Mathematical and Physical Sciences, University of Sussex, ⁵Department of Physics and Material Science, City University of Hong Kong, ⁶State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Science, ⁷Centre for Micro Photonics, Swinburne University of Technology, ⁸Institute of Photonics, Department of Physics, University of Strathclyde, ⁹Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, ¹⁰National Research University of Information Technologies, Mechanics and Optics A protocol is presented for the practical generation and coherent manipulation of high-dimensional frequency-bin entangled photon states using integrated micro-cavities and standard telecommunications components, respectively.

• Medicine

  
  Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability Christina Wayman*^1,2, Denise A. Duricki*^1,2, Lisa A. Roy³, Barbara Haenzi¹, Shi-Yen Tsai⁴, Gwendolyn Kartje^4,5,6, John S. Beech⁷, Diana Cash², Lawrence Moon^{1 1}Department of Neuroimaging, James Black Centre, Institute of Psychiatry, King's College London, University of London, ³Institute of Neuroscience and Psychology, Wellcome Surgical Institute, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, ⁴Research Service, Edward Hines Jr. VA Hospital, ⁵Neurology Service, Edward Hines Jr. VA Hospital, ⁶Department of Molecular Pharmacology and Therapeutics, Neuroscience Research Institute, Loyola University Chicago, ⁷Department of Oncology, The Gray Institute for Radiation, Oncology and Biology, University of Oxford Here we present a protocol to produce permanent distal middle cerebral artery occlusion in elderly female rats with simultaneous occlusion of the carotid arteries to generate large cortical infarcts and sustained deficits. We show confirmation of the lesion size using structural MRI at 24 hr and 8 weeks after stroke.

• Neuroscience

  
  Thermal Imaging to Study Stress Non-invasively in Unrestrained Birds Paul Jerem¹, Katherine Herborn¹, Dominic McCafferty¹, Dorothy McKeegan¹, Ruedi Nager^{1 1}Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow There is a need for a non-invasive assessment of stress. This paper describes a simple protocol using thermal imaging to detect a significant response in eye-region temperature in wild blue tits to a mild acute stressor.

• Bioengineering

  
  Sandwich-like Microenvironments to Harness Cell/Material Interactions José Ballester-Beltrán^1,2, Myriam Lebourg^1,3, Manuel Salmerón-Sánchez^{2 1}Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, ²Division of Biomedical Engineering, School of Engineering, University of Glasgow, ³Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.

• Engineering

  
  Simulation, Fabrication and Characterization of THz Metamaterial Absorbers James P. Grant¹, Iain J.H. McCrindle¹, David R.S. Cumming^{1 1}School of Engineering, University of Glasgow This protocol outlines the simulation, fabrication and characterization of THz metamaterial absorbers. Such absorbers, when coupled with an appropriate sensor, have applications in THz imaging and spectroscopy.

• Bioengineering

  
  Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context Paul Timpson¹, Ewan J. Mcghee¹, Zahra Erami¹, Max Nobis¹, Jean A. Quinn², Mike Edward², Kurt I. Anderson^{1 1}The Beatson Institute for Cancer Research, University of Glasgow, ²Section of Dermatology, School of Medicine, University of Glasgow A method is described for the preparation of a 3-dimensional matrix consisting of collagen type I and primary human fibroblasts. This organotypic gel serves as a useful substrate to assess invasive cell migration because it mimics basic features of tissue stroma and is amenable to many forms of microscopy.