University of Ioannina 2 articles published in JoVE Developmental Biology Detection of DNA Double-Stranded Breaks in Mouse Oocytes Chrysoula Zorzompokou1,2, Marios Ipeirotis1,2, Marios Kaisar Martzoukos1, Petros Marangos1,2 1Department of Biological Applications and Technology, University of Ioannina, 2Biomedical Research Institute, Foundation for Research and Technology Maintaining oocyte genome integrity is necessary to ensure the genetic fidelity in the resulting embryo. Here, we present an accurate protocol for detecting DNA double-strand breaks in mammalian female germ cells. Developmental Biology Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration Sandra Guadalupe Gonzalez Malagon*1,2, Lisa Dobson*1,3, Anna M Lopez Muñoz1, Marcus Dawson1, William Barrell1,3, Petros Marangos2,4, Matthias Krause3, Karen J Liu1 1 This protocol describes the dissection and culture of cranial neural crest cells from mouse models, primarily for the study of cell migration. We describe the live imaging techniques used and the analysis of speed and cell shape changes.