Eotvos Lorand University 6 articles published in JoVE Developmental Biology Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes Jeremy P. Goering*1, Dona Greta Isai*1, Andras Czirok1,2, Irfan Saadi1 1Department of Anatomy and Cell Biology, University of Kansas Medical Center, 2Department of Biological Physics, Eotvos University We present a protocol for isolation and culture of primary mouse embryonic palatal mesenchymal cells for time-lapse imaging of two-dimensional (2D) growth and wound-repair assays. We also provide the methodology for analysis of the time-lapse imaging data to determine cell-stream formation and directional motility. Behavior Comparative Analysis of Lower Limb Kinematics between the Initial and Terminal Phase of 5km Treadmill Running Wenjing Quan1, Meizi Wang2, Gongju Liu2, Gusztáv Fekete3, Julien S. Baker4, Feng Ren1, Yaodong Gu1 1Faculty of Sports Science, Ningbo University, 2Faculty of Engineering, University of Pannonia Veszeprem, 3Savaria Institute of Technology, Eötvös Loránd University, 4Department of Sport, and Physical Education, Hong Kong Baptist University This study investigated the biomechanical characteristics of the lower extremity kinematic variables between the initial and terminal phase of 5 km treadmill running. The lower-limb kinematic data of 10 runners were collected using a three-dimensional motion capture system on a treadmill at the initial phase (0.5 km) and the terminal phase (5 km), respectively. Behavior Biomechanical Analysis Methods to Assess Professional Badminton Players' Lunge Performance Ping Huang1,2,3, Lin Fu1, Yan Zhang 3, Gusztáv Fekete4, Feng Ren1, Yaodong Gu1,2 1Faculty of Sports Science, Ningbo University, 2Research Academy of Grand Health Interdisciplinary, Ningbo University, 3Department of Automation, Biomechanics and Mechatronics, The Lodz University of Technology, 4Savaria Institute of Technology, Eötvös Loránd University Here, we present a protocol to evaluate the differences in injury mechanisms between professional and amateur players when performing a badminton maximal right lunge movement by analyzing lower limb kinematics. Behavior Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements Csilla Ari1, Dominic P. D’Agostino2,3, David M. Diamond1,2, Mark Kindy4,5,6, Collin Park1, Zsolt Kovács7 1Department of Psychology, Hyperbaric Neuroscience Research Laboratory, University of South Florida, 2Department of Molecular Pharmacology and Physiology, Metabolic Medicine Research Laboratory, Morsani College of Medicine, University of South Florida, 3Institute for Human and Machine Cognition, 4Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, 5James A. Haley VA Medical Center, 6Shriners Hospital for Children, 7Savaria Department of Biology, ELTE Eötvös Loránd University Here, we present a protocol to investigate changes in the anxiety level of rodent animal models. The elevated plus maze (EPM) test, used together with a video tracking software, provides a reliable method to document the effect of various potential anxiolytic treatments in preclinical laboratory scenarios. Behavior Using Gold-standard Gait Analysis Methods to Assess Experience Effects on Lower-limb Mechanics During Moderate High-heeled Jogging and Running Yan Zhang 1,2,3, Meizi Wang1, Jan Awrejcewicz3, Gusztáv Fekete4, Feng Ren1, Yaodong Gu1,2 1Faculty of Sports Science, Ningbo University, 2Research Academy of Grand Health Interdisciplinary, Ningbo University, 3Department of Automation, Biomechanics and Mechatronics, The Lodz University of Technology, 4Savaria Institute of Technology, Eötvös Loránd University This study investigated lower-limb kinematics and ground reaction force (GRF) during moderate high-heeled jogging and running. Subjects were divided into groups of experienced wearers and inexperienced wearers. A three-dimensional motion analysis system with a configured force platform captured lower-limb joint movements and GRF. Cancer Research In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues József Murányi1,2, Attila Varga1, Bianka Gurbi1, Pál Gyulavári1, Gábor Mező3, Tibor Vántus2 1MTA-SE Pathobiochemistry Research Group, Semmelweis University, 2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 3MTA-ELTE Research Group of Peptide Chemistry, Eötvös Loránd University Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.
