3 articles published in JoVE
Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements Csilla Ari1, Dominic P. D’Agostino2,3, David M. Diamond1,2, Mark Kindy4,5,6, Collin Park1, Zsolt Kovács7 1Department of Psychology, Hyperbaric Neuroscience Research Laboratory, University of South Florida, 2Department of Molecular Pharmacology and Physiology, Metabolic Medicine Research Laboratory, Morsani College of Medicine, University of South Florida, 3Institute for Human and Machine Cognition, 4Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, 5James A. Haley VA Medical Center, 6Shriners Hospital for Children, 7Savaria Department of Biology, ELTE Eötvös Loránd University Here, we present a protocol to investigate changes in the anxiety level of rodent animal models. The elevated plus maze (EPM) test, used together with a video tracking software, provides a reliable method to document the effect of various potential anxiolytic treatments in preclinical laboratory scenarios.
Using Gold-standard Gait Analysis Methods to Assess Experience Effects on Lower-limb Mechanics During Moderate High-heeled Jogging and Running Yan Zhang1,2,3, Meizi Wang1, Jan Awrejcewicz3, Gusztáv Fekete4, Feng Ren1, Yaodong Gu1,2 1Faculty of Sports Science, Ningbo University, 2Research Academy of Grand Health Interdisciplinary, Ningbo University, 3Department of Automation, Biomechanics and Mechatronics, The Lodz University of Technology, 4Savaria Institute of Technology, Eötvös Loránd University This study investigated lower-limb kinematics and ground reaction force (GRF) during moderate high-heeled jogging and running. Subjects were divided into groups of experienced wearers and inexperienced wearers. A three-dimensional motion analysis system with a configured force platform captured lower-limb joint movements and GRF.
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues József Murányi1,2, Attila Varga1, Bianka Gurbi1, Pál Gyulavári1, Gábor Mező3, Tibor Vántus2 1MTA-SE Pathobiochemistry Research Group, Semmelweis University, 2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 3MTA-ELTE Research Group of Peptide Chemistry, Eötvös Loránd University Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.