Agricultural Research Organization, Volcani Center

3 articles published in JoVE

• Genetics

  
  Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa Shahar Cohen¹, Maxim Itkin¹, Adi Faigenboim¹, Rachel Davidovich-Rikanati², Einat Bar², Daniel Hasson¹, Nurit Shalev³, Hinanit Koltai³, Oded Sagee⁴, Efraim Lewinsohn², Ben Spitzer-Rimon³, Arthur A. Schaffer^{1 1}Department of Vegetable Research, ARO-Volcani Center, ²Department of Vegetable Research, ARO-Newe Ya‘ar Center, ³Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences, ARO- Volcani Center, ⁴Department of Fruit Science, ARO- Volcani Center A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.

• Biology

  
  Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy Dana Charuvi^1,3, Reinat Nevo¹, Ifat Kaplan-Ashiri², Eyal Shimoni², Ziv Reich^{1 1}Department of Biological Chemistry, Weizmann Institute of Science, ²Department of Chemical Research Support, Weizmann Institute of Science, ³Institute of Plant Sciences, Agricultural Research Organization, Volcani Center Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.

• Immunology and Infection

  
  Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues Adi Kliot^1,2, Svetlana Kontsedalov¹, Galina Lebedev¹, Marina Brumin¹, Pakkianathan Britto Cathrin¹, Julio Massaharu Marubayashi¹, Marisa Skaljac^1,3, Eduard Belausov⁴, Henryk Czosnek², Murad Ghanim^{1 1}Department of Entomology, Volcani Center, ²Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, ³Department of Applied Sciences, Institute for Adriatic Crops and Karst Reclamation, ⁴The Institute of Plant Sciences, Volcani Center We describe here a simple fluorescence in situ hybridization (FISH) method for the localization of viruses and bacteria in insect and plant tissues. This protocol can be extended for the visualization of mRNA in whole mount and microscopic sections.