2 articles published in JoVE
Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study Monica Ferro*1, Franca Castiglione*1, Carlo Punta1, Lucio Melone1,2, Walter Panzeri3, Barbara Rossi4, Francesco Trotta5, Andrea Mele1 1Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, 2Università degli studi e-Campus, 3ICRM, CNR - Consiglio Nazionale delle Ricerche, 4Elettra Sincrotrone Trieste, 5Department of Chemistry, University of Torino The motion regimes of ibuprofen encapsulated in β-cyclodextrin nanosponges polymer network are investigated using pulsed-field-gradient spin-echo (PGSE) NMR technique. Synthesis, purification, drug loading, implementation of the NMR pulse sequence and data analysis to work out the mean square displacement of the drug at several observation times are described in detail.
Analysis of Oxidative Stress in Zebrafish Embryos Vera Mugoni1, Annalisa Camporeale1, Massimo M. Santoro1,2 1Department of Molecular Biotechnology and Health Science, University of Torino, 2Laboratory of Endothelial Molecular Biology, Vesalius Research Center, VIB Here we report a protocol to measure oxidative stress in living zebrafish embryos. This procedure allows reactive oxygen species (ROS) detection in both whole embryo tissues and single-cell populations. This protocol will accomplish both qualitative and quantitative analyses.