University of Verona 6 articles published in JoVE Immunology and Infection Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay Tobia Fantoni*1, Michele Bissoli*1, Chiara Stefani1, Mauro Voi1, Alexandrina Dabija1, Rebecca Casula1, Domenico Luca Minafra1, Julys da Fonseca Palmeira2, Enrique Roberto Argañaraz2, Martin Mayora-Neto3, Nigel J. Temperton3, Donato Zipeto1, Alessandra Ruggiero1 1Department of Neuroscience, Biomedicine and Movement, University of Verona, 2Laboratory of Molecular Neurovirology, Faculty of Health Science, University of Brasília, 3Viral Pseudotype Unit, Medway School of Pharmacy, Universities of Kent and Greenwich at Medway Pseudotyped viruses (PVs) are replication-defective virions that are used to study host-virus interactions under safer conditions than handling authentic viruses. Presented here is a detailed protocol that shows how SARS-CoV-2 PVs can be used to test the neutralizing ability of patients' serum after COVID-19 vaccination. Bioengineering Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes Mattia Santoni*1, Edoardo Bertini*1, Roberta Zampieri1, Anna Cuccurullo1, Mauro Commisso1, Elisa Gecchele1, Linda Avesani1 1Department of Biotechnology, University of Verona Here, we present a protocol to produce an oral vaccine candidate against Type 1 diabetes in an edible plant. Biology Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine Pietro Ariani*1, Elodie Vandelle*1, Darren Wong2, Alejandro Giorgetti1, Andrea Porceddu3, Salvatore Camiolo3, Annalisa Polverari1 1Dipartimento di Biotecnologie, Università degli Studi di Verona, 2Ecology and Evolution, Research School of Biology, The Australian National University, 3Dipartimento di Agraria, SACEG, Università degli Studi di Sassari This article describes the procedure for the identification and characterization of a gene family in grapevine applied to the family of Arabidopsis Tóxicos in Levadura (ATL) E3 ubiquitin ligases. Immunology and Infection An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses Gabriella Bergamini*1, Fabio Stellari*2, Angela Sandri*3, Maria M. Lleo3, Gaetano Donofrio4, Francesca Ruscitti5,2, Federico Boschi6, Andrea Sbarbati7, Gino Villetti2, Paola Melotti8, Claudio Sorio1 1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, 2Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division, University of Verona, 4Dipartimento di Scienze Medico-Veterinarie, University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences, University of Parma, 6Department of Computer Science, University of Verona, 7Department of Neurological, Biomedical and Movement Sciences, University of Verona, 8Cystic Fibrosis Regional Center (CFC), AOUI Verona The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct. Genetics The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics Silvia Dal Santo*1, Mauro Commisso*1, Erica D'Incà1, Andrea Anesi1,2, Matteo Stocchero3, Sara Zenoni1, Stefania Ceoldo1, Giovanni B. Tornielli1, Mario Pezzotti1, Flavia Guzzo1 1Biotechnology Department, University of Verona, 2Lab. of Bioorganic Chemistry, Physics Department, University of Trento, 3S-IN Soluzioni Informatiche This article describes the application of untargeted metabolomics, transcriptomics and multivariate statistical analysis to grape berry transcripts and metabolites in order to gain insight into the terroir concept, i.e., the impact of the environment on berry quality traits. Biology A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems Elisa Gecchele*1, Matilde Merlin*1, Annalisa Brozzetti2, Alberto Falorni2, Mario Pezzotti1, Linda Avesani1 1Department of Biotechnology, University of Verona, Verona, Italy, 2Department of Internal Medicine, University of Perugia, Perugia, Italy In this study the expression of a target human recombinant protein in different production platforms was compared. We focused on traditional fermenter-based cultures and on plants, describing the set-up of each system and highlighting, on the basis of the reported results, the inherent limits and advantages for each platform.
