3 articles published in JoVE
Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing Maria Felicia Soluri1, Simone Puccio2, Giada Caredda2, Giorgio Grillo3, Vito Flavio Licciulli3, Arianna Consiglio3, Paolo Edomi4, Claudio Santoro1, Daniele Sblattero4, Clelia Peano5,6 1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy The protocols described allow the construction, characterization and selection (against the target of choice) of a "domainome" library made from any DNA source. This is achieved by a research pipeline that combines different technologies: phage display, a folding reporter and next generation sequencing with a web tool for data analysis.
Low Pressure Vapor-assisted Solution Process for Tunable Band Gap Pinhole-free Methylammonium Lead Halide Perovskite Films Carolin M. Sutter-Fella*1,2,3, Yanbo Li*1,4, Nicola Cefarin1,5,6, Aya Buckley1,7, Quynh Phuong Ngo8,9, Ali Javey2,3, Ian D. Sharp1, Francesca M. Toma1 1Joint Center for Artificial Photosynthesis, Chemical Sciences Division, Lawrence Berkeley National Laboratory, 2Electrical Engineering and Computer Sciences, University of California, Berkeley, 3Materials Science Division, Lawrence Berkeley National Laboratory, 4Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 5Department of Physics, Graduate School of Nanotechnology, University of Trieste, 6TASC Laboratory, IOM-CNR - Istituto Officina dei Materiali, 7Department of Chemistry, University of California, Berkeley, 8Materials Science and Engineering, University of California, Berkeley, 9Joint Center for Artificial Photosynthesis, Lawrence Berkeley National Laboratory Here, we present a protocol for the synthesis of CH3NH3I and CH3NH3Br precursors and the subsequent formation of pinhole-free, continuous CH3NH3PbI3-xBrx thin films for the application in high efficiency solar cells and other optoelectronic devices.
Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts Deborah Mattinzoli1, Piergiorgio Messa2, Alessandro Corbelli1, Masami Ikehata1, Anna Mondini1, Cristina Zennaro3, Silvia Armelloni1, Min Li1, Laura Giardino1, Maria Pia Rastaldi1 1 Treatment of primary mouse osteoblasts with retinoic acid produces a homogeneous population of ramified cells bearing morphological and molecular features of osteocytes. The method overcomes the difficulty of obtaining and maintaining primary osteocytes in culture, and can be advantageous to study cells derived from transgenic models.