University of Modena and Reggio Emilia 5 articles published in JoVE Medicine Platelet-Rich Plasma Lysate for Treatment of Eye Surface Diseases Lucia Merolle1, Barbara Iotti1, Pamela Berni1, Elisa Bedeschi1, Katya Boito1, Eleonora Maurizi2, Gaia Gavioli1, Agnese Razzoli1,3, Roberto Baricchi1, Chiara Marraccini1, Davide Schiroli1 1Transfusion Medicine Unit, AUSL-IRCCS di Reggio Emilia, 2Interdepartmental Centre for Regenerative Medicine “Stefano Ferrari”, University of Modena and Reggio Emilia, 3Clinical and Experimental Medicine PhD Program, University of Modena and Reggio Emilia Platelet lysates represent an emerging tool for the treatment of ocular surface diseases. Here, we propose a method for the preparation, dispensation, storage, and characterization of platelet lysate collected from platelet donors. Biology An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector Daniela Benati1, Fabienne Cocchiarella1, Alessandra Recchia1 1Centre for Regenerative Medicine, Department of Life Sciences, University of Modena and Reggio Emilia This protocol describes a method to achieve stable integration of a gene of interest into the human genome by the transcriptionally regulated Sleeping Beauty system. Preparation of the integration of defective lentiviral vectors, the in vitro transduction of human cells, and the molecular assay on transduced cells are reported. Biology In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells Francesco Castagnetti1,2, Elisabetta Fiacco3, Carol Imbriano4, Lucia Latella1,2 1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice. Genetics Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions Oriana Romano1,2, Ingrid Cifola3, Valentina Poletti4, Marco Severgnini3, Clelia Peano3, Gianluca De Bellis3, Fulvio Mavilio1,4, Annarita Miccio1,2,4,5 1Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, 3Institute for Biomedical Technologies, CNR, 4Généthon, 5Sorbonne Paris Cité - Université Paris Descartes Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells. Medicine Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs Alessandro Di Cerbo1, Federica Pezzuto2, Sergio Canello3, Gianandrea Guidetti3, Beniamino Palmieri1,4 1Department of Surgery and Surgical Specialties, Azienda Ospedaliero-Universitaria Policlinico di Modena, 2Department of Clinical Microbiology, Universtity of Modena and Reggio Emilia, 3Sanypet S.p.a, 4AIRMO Center Milan We describe a simple approach for diagnosis of halitosis in dogs as well as a dietary approach for its management. This protocol may be extended to the management of halitosis in humans in the near future.
