University of Ferrara 6 articles published in JoVE Medicine Functional Characterization of Endogenously Expressed Human RYR1 Variants Susan Treves1,2, Thierry Girard3, Francesco Zorzato1,2 1Department of Biomedicine, Basel University Hospital, 2Department of Life Sciences, University of Ferrara, 3Department of Anesthesia, Basel University Hospital Here methods used to study the functional effect of RYR1 mutations endogenously expressed in Epstein Barr Virus immortalized human B-lymphocytes and muscle biopsy derived satellite cells differentiated into myotubes are described. Bioengineering Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale Irini M. Dijkhoff1, Benedetta Petracca1,2, Roxane Prieux3, Giuseppe Valacchi3,4, Barbara Rothen-Rutishauser1, Marc Eeman2 1Adolphe Merkle Institute, University of Fribourg, 2Dow Silicones Belgium SRL, 3Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 4Department of Animal Sciences, Plants for Human Health Institute, North Carolina State University This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented. Biology Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization Bianca Vezzani1,2, Mario Gomez-Salazar1, Joan Casamitjana1, Carlo Tremolada3, Bruno Péault1,4 1MRC Center for Regenerative Medicine, University of Edinburgh, 2Dept. of Morphology, Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara, 3Italian Image Institute, 4Orthopaedic Hospital Research Center and Broad Stem Cell Research Center, David Geffen School of Medicine, University of California Here, we present human adipose tissue enzyme-free micro-fragmentation using a closed system device. This new method allows the obtainment of sub-millimeter clusters of adipose tissue suitable for in vivo transplantation, in vitro culture, and further cell isolation and characterization. Neuroscience Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats Marie Soukupová1, Chiara Falcicchia1, Francesca Lovisari1, Selene Ingusci1, Mario Barbieri1, Silvia Zucchini1, Michele Simonato1 1Department of Medical Sciences, Section of Pharmacology, Neuroscience Center, University of Ferrara and National Institute of Neuroscience Here, we describe a method for in vivo microdialysis to analyze aspartate and glutamate release in the ventral hippocampus of epileptic and non-epileptic rats, in combination with EEG recordings. Extracellular concentrations of aspartate and glutamate may be correlated with the different phases of the disease. Neuroscience Personalized Needles for Microinjections in the Rodent Brain Giovanna Paolone*1, Chiara Falcicchia*1, Gianluca Verlengia*1, Mario Barbieri1, Anna Binaschi1, Federico Paliotto1, Beatrice Paradiso1, Marie Soukupova1, Silvia Zucchini1,2, Michele Simonato1,2 1Department of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara, 2Laboratory for the Technologies for Advanced Therapies (LTTA), University of Ferrara We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used. Bioengineering Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR Manuela Ferracin1, Irene Salamon2, Laura Lupini2, Elena Miotto2, Silvia Sabbioni3, Massimo Negrini4 1Department of Experimental, Diagnostic and Specialty Medicine - DIMES, University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 3Department of Life Sciences and Biotechnology, University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.
