3 articles published in JoVE
Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats Marie Soukupová1, Chiara Falcicchia1, Francesca Lovisari1, Selene Ingusci1, Mario Barbieri1, Silvia Zucchini1, Michele Simonato1 1Department of Medical Sciences, Section of Pharmacology, Neuroscience Center, University of Ferrara and National Institute of Neuroscience Here, we describe a method for in vivo microdialysis to analyze aspartate and glutamate release in the ventral hippocampus of epileptic and non-epileptic rats, in combination with EEG recordings. Extracellular concentrations of aspartate and glutamate may be correlated with the different phases of the disease.
Personalized Needles for Microinjections in the Rodent Brain Giovanna Paolone*1, Chiara Falcicchia*1, Gianluca Verlengia*1, Mario Barbieri1, Anna Binaschi1, Federico Paliotto1, Beatrice Paradiso1, Marie Soukupova1, Silvia Zucchini1,2, Michele Simonato1,2 1Department of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara, 2Laboratory for the Technologies for Advanced Therapies (LTTA), University of Ferrara We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used.
Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR Manuela Ferracin1, Irene Salamon2, Laura Lupini2, Elena Miotto2, Silvia Sabbioni3, Massimo Negrini4 1Department of Experimental, Diagnostic and Specialty Medicine - DIMES, University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 3Department of Life Sciences and Biotechnology, University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.