3 articles published in JoVE
Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery Chiara Pagliuca1, Elena Scaglione1, Francesca Carraturo1, Giuseppe Mantova1, Maria Michela Marino1, Maria Virginia Pishbin1, Caterina Pagliarulo2, Roberta Colicchio1, Paola Salvatore1,3 1Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 2Department of Science and Technology, Sannio University, 3CEINGE – Advanced Biotechnology Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.
Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens Rachele Isticato1, Ezio Ricca1, Loredana Baccigalupi2 1Department of Biology, Federico II University, 2Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II" This protocol focuses on the use of bacterial spores as a "live" nanobiotechnological tool to adsorb heterologous molecules with various biological activities. The methods to measure the efficiency of adsorption are also shown.
In Vitro Enzyme Measurement to Test Pharmacological Chaperone Responsiveness in Fabry and Pompe Disease Jan Lukas1, Anne-Marie Knospe1, Susanne Seemann1, Valentina Citro2, Maria V. Cubellis2, Arndt Rolfs1,3 1Albrecht-Kossel-Institute, University Rostock Medical Center, 2Department of Biology, University Federico II, 3Centogene AG There is a demand to make pre-clinical testing for a novel class of "orphan" drugs called pharmacological chaperones reproducible, fast, and efficient. We developed a simple, highly standardized, and versatile cell culture-based assay to screen for eligible patients as well as novel pharmacological chaperone drugs.