Centro Cardiologico Monzino-IRCCS

1 article published in JoVE

• Developmental Biology

  
  Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients Chiara Assunta Pilato*^1,2, Ilaria Stadiotti*¹, Angela Serena Maione¹, Valentina Saverio¹, Valentina Catto³, Fabrizio Tundo³, Antonio Dello Russo³, Claudio Tondo^2,3, Giulio Pompilio^1,2, Michela Casella*³, Elena Sommariva*^{1 1}Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino-IRCCS, ²Department of Clinical Sciences and Community Health, Università degli Studi di Milano, ³Heart Rhythm Center, Centro Cardiologico Monzino-IRCCS In this article, a method to isolate cardiac mesenchymal stromal cells from endomyocardial bioptic samples of arrhythmogenic cardiomyopathy patients is provided. Their characterization and the protocol to boost their adipogenic differentiation are described.