2 articles published in JoVE
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene Vincenzo De Iuliis1, Sebastiano Ursi1, Alfonso Pennelli2, Marika Caruso2, Sabrina Capodifoglio2, Antonio Marino1, Vincenzo Flati3, Gianfranco Vitullo1, Elena Toniato2, Iole Robuffo4, Stefano Martinotti1,2 1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences, University of Chieti, 3 In the present study, we describe a methodology to analyze the C924T genotype. The protocol consists of three phases: DNA extraction, amplification by polymerase chain reaction (PCR), and analysis of the restriction fragment length polymorphism (RFLP) on agarose gel.
Slow-release Drug Delivery through Elvax 40W to the Rat Retina: Implications for the Treatment of Chronic Conditions Lavinia Fiorani*1, Rita Maccarone*1, Nilisha Fernando2,3, Linda Colecchi1, Silvia Bisti1,2, Krisztina Valter2,3,4 1 This paper details how Elvax 40W can be used as a slow-release method for drug delivery to the adult rat retina. The protocol for preparing, loading, and delivering the drug-resin complex to the eye is described.