Universita del Piemonte Orientale & IRCAD 2 articles published in JoVE Medicine Manufacture of a Multi-Purpose Low-Cost Animal Bench-Model for Teaching Tracheostomy Federico Sacco Botto1, Pier Luigi Ingrassia2, Paolo Donato3, Massimiliano Garzaro4, Paolo Aluffi4, Sergio Gentilli5, Massimo Olina6, Elena Grossini1 1Physiology and Experimental Surgery, Department of Translational Medicine, Università del Piemonte Orientale, 2Centro Interdipartimentale di Didattica Innovativa e di Simulazione in Medicina e Professioni Sanitarie - SIMNOVA, Università del Piemonte Orientale, 3Section of Anesthesia and Intensive Care, Azienda Ospedaliera Maggiore della Carità, Università del Piemonte Orientale, 4Section of ENT, Azienda Ospedaliera Maggiore della Carità, Department of Health Sciences, Università del Piemonte Orientale, 5Section of General Surgery Department of Health of Sciences, Azienda Ospedaliera Maggiore della Carità, Università del Piemonte Orientale, 6Department of Education, Azienda Ospedaliera Maggiore della Carità This article illustrates every step of the manufacture of a new multi-purpose low-cost animal bench-model for subglottic airway access management. All the procedures are shown in the video. The model's realism and its suitability for training the given clinical maneuvers were assessed by independent senior otolaryngologists and anesthesiologists. Biology Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing Maria Felicia Soluri1, Simone Puccio2, Giada Caredda2, Giorgio Grillo3, Vito Flavio Licciulli3, Arianna Consiglio3, Paolo Edomi4, Claudio Santoro1, Daniele Sblattero4, Clelia Peano5,6 1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy The protocols described allow the construction, characterization and selection (against the target of choice) of a "domainome" library made from any DNA source. This is achieved by a research pipeline that combines different technologies: phage display, a folding reporter and next generation sequencing with a web tool for data analysis.
