2 articles published in JoVE
Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters Michele Caccia1, Simone Caglio1, Anna Galli1,2, Matteo Interlenghi2, Isabella Castiglioni2,3, Marco Martini1 1Dipartimento di Scienza dei Materiali, Università degli Studi di Milano-Bicocca, 2CNR-IBFM, 3Dipartimento di Fisica, Università degli Studi di Milano-Bicocca Hyperspectral Reflectance Imaging hypercubes include remarkable information into a large amount of data. Therefore, the request for automated protocols to manage and study the datasets is widely justified. The combination of Spectral Angle Mapper, data manipulation, and a user-adjustable analysis method constitutes a key-turn for exploring the experimental results.
Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing Maria Felicia Soluri1, Simone Puccio2, Giada Caredda2, Giorgio Grillo3, Vito Flavio Licciulli3, Arianna Consiglio3, Paolo Edomi4, Claudio Santoro1, Daniele Sblattero4, Clelia Peano5,6 1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy The protocols described allow the construction, characterization and selection (against the target of choice) of a "domainome" library made from any DNA source. This is achieved by a research pipeline that combines different technologies: phage display, a folding reporter and next generation sequencing with a web tool for data analysis.