Istituto Superiore di Sanita 3 articles published in JoVE Biology High-Quality Brain and Bone Marrow Nuclei Preparation for Single Nuclei Multiome Assays Carolina Moraes Cabé1, Sophie Novault1, Ana Jeemin Choi2, Valerie Seffer1, Laura Barrio Cano1, Valentina Libri1,3, Milena Hasan1 1Cytometry and Biomarkers UTechS, C2RT, Institut Pasteur, Université Paris Cité, 2Microenvironment and Immunity Unit, Institut Pasteur, Université Paris Cité, INSERM U1224, 3Istituto Superiore di Sanità The success of single-cell/single-nuclei transcriptomics and multi-omics largely depends on the quality of cells/nuclei. Therefore, isolating cells/nuclei from tissue and their purification must be highly standardized. This protocol describes the preparation of nuclei from the brain and bone marrow for downstream single-nuclei multiome assay. Immunology and Infection Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments Adele De Ninno1, Francesca Romana Bertani1, Annamaria Gerardino1, Giovanna Schiavoni2, Martina Musella3, Claudia Galassi3, Fabrizio Mattei2, Antonella Sistigu3,4, Luca Businaro1 1CNR Institute for Photonics and Nanotechnology, 2Dept. of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 3Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, 4Tumor Immunology and Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute In the age of immunotherapy and single-cell genomic profiling, cancer biology requires novel in vitro and computational tools for investigating the tumor-immune interface in a proper spatiotemporal context. We describe protocols to exploit tumor-immune microfluidic co-cultures in 2D and 3D settings, compatible with dynamic, multiparametric monitoring of cellular functions. Immunology and Infection Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples Anne Mayer-Scholl1, Edoardo Pozio2, Jennifer Gayda1, Nora Thaben1, Peter Bahn1, Karsten Nöckler1 1Federal Institute for Risk Assessment, 2Istituto Superiore di Sanità The prevention of human trichinellosis in many countries worldwide is based on the laboratory examination of muscle samples from susceptible animals by methods of digestion, of which the magnetic stirrer method is considered the gold standard.
