Fondazione Bruno Kessler 1 article published in JoVE Neuroscience Peering at Brain Polysomes with Atomic Force Microscopy Lorenzo Lunelli1, Paola Bernabò2, Alice Bolner2, Valentina Vaghi1, Marta Marchioretto2, Gabriella Viero2 1Laboratory of Biomolecular Sequence and Structure Analysis for Health, Fondazione Bruno Kessler, 2Institute of Biophysics, CNR Unit at Trento While ribosome structure has been extensively characterized, the organization of polysomes is still understudied. To overcome this lack of knowledge, we present here a detailed preparation protocol for accurate imaging of mammalian polysomes by atomic force microscopy (AFM) in air and liquid.
