Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR 1 article published in JoVE Developmental Biology Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection Ombretta Guardiola1, Gennaro Andolfi1, Mario Tirone2,3, Francescopaolo Iavarone1, Silvia Brunelli2, Gabriella Minchiotti1 1Stem Cell Fate Laboratory, Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR, 2School of Medicine and Surgery, University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute This manuscript describes a detailed protocol to induce acute skeletal muscle regeneration in adult mice and subsequent manipulations of the muscles, such as dissection, freezing, cutting, routine staining, and myofiber cross-sectional area analysis.
