International Center of Computational Neurophotonics 1 article published in JoVE Biology Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions Carina Monico1,2, Gionata Belcastro1, Francesco Vanzi1,3, Francesco S. Pavone1,4,5,6, Marco Capitanio1,4 1LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, 2Chemistry Research Laboratory, University of Oxford, 3Department of Biology, University of Florence, 4Department of Physics and Astronomy, University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.
