2 articles published in JoVE
Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders Giovanni Provenzano1, Luca Pangrazzi1, Andrea Poli2, Nicoletta Berardi2, Yuri Bozzi1,2 1Centre for Integrative Biology (CIBIO), University of Trento, 2CNR Neuroscience Institute, Pisa, Italy We describe an immunohistochemistry protocol to study the profile of hippocampal neuron activation following exposure to a spatial learning task in a mouse model characterized by cognitive deficits of neurodevelopmental origin. This protocol can be applied to both genetic or pharmacological mouse models characterized by cognitive deficits.
Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells Matteo Fossati1,2,3, Nica Borgese2,3,4, Sara Francesca Colombo2,3, Maura Francolini1,2 1Fondazione Filarete, 2Department of Biotechnology and Translational Medicine, University of Milan, 3Neuroscience Institute, National Research Council (CNR), 4Department of Health Science, "Magna Graecia" University of Catanzaro We describe the imaging approaches we use to investigate the distribution and mobility of the transfected fluorescent proteins resident in the endoplasmic reticulum (ER) by means of the confocal imaging of living cells. We also ultrastructurally analyze the effect of their expression on the architecture of this subcellular compartment.