IRCCS - Istituto di Ricerche Farmacologiche Mario Negri 3 articles published in JoVE Medicine Transthoracic Echocardiography to Assess Post-Resuscitation Left Ventricular Dysfunction After Acute Myocardial Infarction and Cardiac Arrest in Pigs Daria De Giorgio1, Davide Olivari1, Francesca Fumagalli1, Lidia Staszewsky1, Giuseppe Ristagno2,3 1Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2University of Milan, 3 Transthoracic echocardiography is the first-line diagnostic test for post-resuscitation left ventricular dysfunction and structural changes in a pig model of cardiac arrest. Neuroscience Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons Luca Russo1, Carmina Natale1, Andrea Conz1, Joe Kelk2, Elena Restelli2, Roberto Chiesa2, Mario Salmona1, Luana Fioriti2,3, Luca Colnaghi1 1Department of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute, Istituto di Ricerche Farmacologiche Mario Negri IRCCS This protocol shows how to employ super-resolution microscopy to study protein co-localization in primary neuronal cultures. Neuroscience Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury Carlo Perego*1, Stefano Fumagalli*1, Maria-Grazia De Simoni1 1Department of Neuroscience, IRCCS - Istituto di Ricerche Farmacologiche Mario Negri A way to gain new insights into the complexity of the brain inflammatory response is presented. We describe immunofluorescence-based protocols followed by three-dimensional confocal analysis to investigate the pattern of co-expression of microglia/macrophage phenotype markers in a mouse model of focal ischemia.
