Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII 1 article published in JoVE Immunology and Infection Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin Fiorenza Orlando*1, Chiara Paolini*2, Silvia Agarbati2, Cecilia Tonnini2, Antonella Grieco2, Chiara Capelli3, Martino Introna3, Mauro Provinciali1, Armando Gabrielli2, Gianluca Moroncini2 1Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA), 2Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, 3UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII Here we present an effective method to investigate the antifibrotic activity of intravenously infused human mesenchymal stromal cells obtained from the whole umbilical cord following the induction of lung injury by an endotracheal injection of bleomycin in C57BL/6 mice. This protocol can be easily extended to the preclinical testing of other therapeutics.
