Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) 4 articles published in JoVE Developmental Biology Quantification of Self-renewal in Murine Mammosphere Cultures Thalia Vlachou1, Xieraili Aobuli1, Errico D'Elia1, Angela Santoro1, Maria Cristina Moroni1, Pier Giuseppe Pelicci1,2 1Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, 2Department of Oncology and Hemato-Oncology, University of Milan Here, we describe the implementation and interpretation of the results of an in vitro mammosphere self-renewal quantitative assay. Immunology and Infection Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion Romana Vidergar*1, Chiara Agostinis*2, Paola Zacchi1, Alessandro Mangogna1, Fleur Bossi2, Fabrizio Zanconati3, Marco Confalonieri3, Giuseppe Ricci2,3, Roberta Bulla1 1Department of Life Sciences, University of Trieste, 2Institute for Maternal and Child Health, IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico) Burlo Garofolo, 3Department of Medical, Surgical and Health Science, University of Trieste The complement component C1q is a pro-inflammatory molecule highly expressed in the tissue microenvironment that can interact with the extracellular matrix. Here, we describe a method to test how C1q bound to hyaluronic acid impacts cell adhesion. Immunology and Infection Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin Fiorenza Orlando*1, Chiara Paolini*2, Silvia Agarbati2, Cecilia Tonnini2, Antonella Grieco2, Chiara Capelli3, Martino Introna3, Mauro Provinciali1, Armando Gabrielli2, Gianluca Moroncini2 1Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA), 2Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, 3UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII Here we present an effective method to investigate the antifibrotic activity of intravenously infused human mesenchymal stromal cells obtained from the whole umbilical cord following the induction of lung injury by an endotracheal injection of bleomycin in C57BL/6 mice. This protocol can be easily extended to the preclinical testing of other therapeutics. Neuroscience Real-time Video Projection in an MRI for Characterization of Neural Correlates Associated with Mirror Therapy for Phantom Limb Pain Faddi G. Saleh Velez1,2, Camila B. Pinto1,3, Emma S. Bailin4, Marionna Münger1, Andrew Ellison5, Beatriz T. Costa1, David Crandell6, Nadia Bolognini7,8, Lotfi B. Merabet4, Felipe Fregni1 1Laboratory of Neuromodulation & Center for Clinical Research Learning, Department of Physical Medicine and Rehabilitation, Harvard Medical School, Spaulding Rehabilitation Hospital, 2University of Chicago Medical Center, Department of Neurology, University of Chicago, 3Department of Neuroscience and Behavior, Psychology Institute, University of Sao Paulo, 4The Laboratory for Visual Neuroplasticity, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 5Center for Biomedical Imaging, Department of Anatomy and Neurobiology, Boston University School of Medicine, 6Spaulding Rehabilitation Hospital, Harvard Medical School, 7Department of Psychology & Milan Center for Neuroscience, University of Milano-Bicocca, 8Neuropsychological Laboratory, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Istituto Auxologico Italiano We present a novel combined behavioral and neuroimaging protocol employing real-time video projection for the purpose of characterizing the neural correlates associated with mirror therapy within the magnetic resonance imaging scanner environment in leg amputee subjects with phantom limb pain.
