Sabina Universitas 1 article published in JoVE Developmental Biology Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells Stefano Martellucci1,2, Costantino Santacroce1, Valeria Manganelli2, Francesca Santilli1,2, Luca Piccoli3, Michele Cassetta3, Roberta Misasi2, Maurizio Sorice2, Vincenzo Mattei1,2 1Laboratory of Experimental Medicine and Environmental Pathology, Sabina Universitas - Rieti University Hub, 2Department of Experimental Medicine, Sapienza University of Rome, 3Department of Science Dentistry and Maxillofacial, Sapienza University of Rome Here we present a protocol for human Dental Pulp Stem Cells isolation and propagation in order to evaluate the prion protein expression during neuronal differentiation process.