University of Sannio 2 articles published in JoVE Immunology and Infection Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery Chiara Pagliuca1, Elena Scaglione1, Francesca Carraturo1, Giuseppe Mantova1, Maria Michela Marino1, Maria Virginia Pishbin1, Caterina Pagliarulo2, Roberta Colicchio1, Paola Salvatore1,3 1Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 2Department of Science and Technology, Sannio University, 3CEINGE – Advanced Biotechnology Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues. Biochemistry Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods Roberta Imperatore1,2, Livia D'Angelo3, Paolo De Girolamo3, Luigia Cristino2, Marina Paolucci1 1Department of Sciences and Technologies (DST), University of Sannio, 2Endocannabinoid Research Group, Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions, University of Naples Federico II Presented here are protocols for immunohistochemical characterization and localization of orexin peptide, orexin receptors, and endocannabinoid receptors in the gut and brains of normal and diet-induced obesity (DIO) adult zebrafish models using immunoperixidase and double immunofluorescence methods.
