Italian Image Institute 1 article published in JoVE Biology Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization Bianca Vezzani1,2, Mario Gomez-Salazar1, Joan Casamitjana1, Carlo Tremolada3, Bruno Péault1,4 1MRC Center for Regenerative Medicine, University of Edinburgh, 2Dept. of Morphology, Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara, 3Italian Image Institute, 4Orthopaedic Hospital Research Center and Broad Stem Cell Research Center, David Geffen School of Medicine, University of California Here, we present human adipose tissue enzyme-free micro-fragmentation using a closed system device. This new method allows the obtainment of sub-millimeter clusters of adipose tissue suitable for in vivo transplantation, in vitro culture, and further cell isolation and characterization.
