Ospedale Pediatrico Bambino Gesu, IRCCS 1 article published in JoVE Biology Measuring the Confluence of iPSCs Using an Automated Imaging System Valentina Magliocca1,2, Maria Vinci3, Tiziana Persichini2, Franco Locatelli3, Marco Tartaglia1, Claudia Compagnucci1 1Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, 2Department of Science, University Roma Tre, 3Department of Onco-hematology, Gene and Cell Therapy, Ospedale Pediatrico Bambino Gesù, IRCCS The goal of the protocol is to compare different extracellular matrix (ECM) coating conditions to assess how differential coating affects the growth rate of induced pluripotent stem cells (iPSCs). In particular, we aim to set up conditions to obtain optimal growth of iPSC cultures.
